Group

Calcein Disodium Salt is a fluorescent dye widely used in biological and biochemical research due to its excellent cell-permeant properties and vivid green fluorescence. It belongs to the family of fluorescein derivatives and is commonly employed as a staining agent, indicator, and tracer in various laboratory applications.

One of the primary uses of Calcein Disodium Salt is in cell viability and cytotoxicity assays. When introduced into live cells, calcein is hydrolyzed by intracellular esterases into its fluorescent form, allowing researchers to distinguish living cells from dead ones easily. This makes it invaluable in studies assessing the effects of drugs, toxins, or environmental conditions on cell health.

Calcein Disodium Salt is also used in calcium imaging and mineralization studies. In bone research, it serves as a fluorescent marker for calcium deposition, enabling scientists to visualize and quantify bone formation in vitro and in vivo. This application is particularly useful in studying osteogenesis and evaluating the performance of biomaterials for bone regeneration.

In addition, Calcein Disodium Salt plays a role in permeability and transport studies. For example, it can help evaluate the integrity of cellular membranes or the functionality of membrane transporters, contributing to drug development and toxicology research.

Thanks to its high sensitivity, low toxicity, and compatibility with fluorescence microscopy and flow cytometry, Calcein Disodium Salt remains a versatile tool in academic and industrial laboratories. Proper handling and storage are important to preserve its fluorescence efficiency, as prolonged exposure to light or improper pH conditions can degrade its performance.

As research in cell biology, regenerative medicine, and material science continues to expand, Calcein Disodium Salt is likely to remain a staple reagent for visualizing and quantifying key cellular processes with precision and ease.